本年の活動のみ表記しています。

過去の論文、学会活動、研究などについての御詳細は各医師、スタッフにお尋ね下さい。（それぞれのスタッフが専門的活動をしております）

１０月はとても学会が多い時期で、職員関係者が出席するため、皆様にご迷惑をおかけします。

10/30　第35回日本心血管インターベンション治療学会関東甲信越地方会

10/１７　第14回東京吸入療法研究会　とくに咳喘息についての知見

10/7　座長を務めました。　Interventional Cardiologyst Conference in Tokyo 抗血小板剤の使用方法

論文受諾されました

Journal of Molecular and Cellular Cardiology

Dedifferentiated fat cells convert to cardiomyocyte phenotype and repair infarcted cardiac tissue  in rats

Abstract: Objective. Adipose tissue-derived stem cells have been demonstrated to differentiate into  cardiomyocytes and vascular endothelial cells. Here we investigate whether mature adipocyte-derived  dedifferentiated fat (DFAT) cells can differentiate to cardiomyocytes in vitro and in vivo by  establishing DFAT cell lines via ceiling culture of mature adipocytes. Methods. DFAT cells were  obtained by dedifferentiation of mature adipocytes from GFP-transgenic rats. We evaluated the  differentiating ability of DFAT cells into cardiomyocytes by detection of the cardiac phenotype markers  in immunocytochemical and RT-PCR analyses in vitro. We also examined effects of the transplantation  of DFAT cells into the infarcted heart of rats on cardiomyocytes regeneration and angiogenesis.  Results. DFAT cells expressed cardiac phenotype markers when cocultured with cardiomyocytes and  also when grown in MethoCult medium in the absence of cardiomyocytes, indicating that DFAT cells  have the potential to differentiate to cardiomyocyte lineage. In a rat acute myocardial infarction model,  transplanted DFAT cells were efficiently accumulated in infarcted myocardium and expressed cardiac  sarcomeric actin at 8 weeks after the cell transplantation. The transplantation of DFAT cells  significantly (p < 0.05) increased capillary density in the infarcted area, when compared with hearts  from saline-injected control rats. Conclusion. We　demonstrated that DFAT cells have the ability to  differentiate to cardiomyocyte-like cells in vitro and in vivo. In addition, transplantation of DFAT cells  led to neovascuralization in rats with myocardial infarction. We propose that DFAT cells represent a  promising candidate cell source for cardiomyocyte regeneration in severe ischemic heart disease.

共同事業

１）長崎大学熱帯医学研究所に渡航時の旅行医学、予防接種や注意事項などについて、様々な助言を頂ける事になりました。

２）国際医療福祉大学三田病院、太田恵一郎先生のご協力により癌治療の指導を頂けるようになりました。

３）葛谷信明先生のご協力により糖尿病を中心とした生活習慣病の予防医学の共同研究を予定しました。

４）AED, BLS(心肺蘇生講習会)について、心臓血管研究所の協賛をいただけることになりました。

講演、発表してきました。

第１回循環器病における糖尿病治療についての座談会

座談形式：池袋

「健康診断と人間ドックの意義」講演してきました。

医療健康教室、東京北部病院において

参加してきました。

TOPIC

参加してきました：渋谷開催

第２９回日本静脈学会

参加してきました；名古屋開催

CVIT

各種シンポジウム、教育講演など：札幌開催

日本糖尿病学会

参加してきました；大阪開催

JET

参加してきました：神戸開催

再生医療学会

不応性虚血性心疾患に対する冠静脈経由の細胞移植療法（臨床応用）再生医療 (1347-7919)巻:８ 頁:179

2000年から基礎研究を経て臨応用に至るまでの再生細胞治療の発表を行いました。

TCIF 2009

冠動脈石灰化病変におけるAngioSculpt scoring balloon Catheter (AngioSculptTM) の有効性の検討 – Optical Coherence Tomography(OCT)による評価

第７３回日本循環器学会: toｔal 16 abstracts

1)Late Breaking Clinical Trials

Evaluation of the Prospective Observation of Erythropoietin Administration for the Treatment od acute Myocardial Infarction (EPO-AMI)

2)Oral Presentation, Regenerative Therapy

Safety of Catheter-based Autologous Bone Marrow Cells Implantation Using a Retrograde Injection Method through Coronary Vein for Therapeutics Angiogenesis

etc.

論文発表

Serum hepcidin-20 is elevated during the acute phase of myocardial infarction. Tohoku J Exp Med. 2009;218(2):93-8.

（Hepcidin-20は心筋梗塞急性期に上昇する）を共同論文発表しました。

Hepcidin, a key iron-regulator secreted from the liver, consists of 25 amino acids (hepcidin-25), blocks iron release from macrophages via internalization and degradation of cellular iron exporter ferroportin, and restrains the use of iron in organs. Hepcidin mRNA and protein are also expressed in the human heart. A short form of hepcidin that lacks 5 amino-acid residues in the N-terminus (hepcidin-20) has been found in human serum, although its physiological role is unknown. Here, we successfully measured the serum levels of hepcidin-25 and hepcidin-20 in 12 patients with acute myocardial infarction (AMI) using surface-enhanced laser desorption ionization time of flight mass spectrometry. Among the selected 10 patients, whose blood samples were taken within 4 hours after a heart attack, all the patients showed elevated serum levels of hepcidin-20 [between 31.7 and 285.1 arbitrary unit (AU); normal level < 9.3 AU], while 8 patients showed high levels of hepcidin-25 (9.3-271.4; normal < 25.5 AU). The hepcidin-20 level was decreased to nearly the normal level on day 7 (range of 2.9 to 12.5 AU) in the 12 patients, whereas the hepcidin-25 level remained high on day 7 in 8 patients. Furthermore, the elevated levels of hepcidin-25 and hepcidin-20 were not correlated with the serum levels of markers for inflammation, interleukin-6 and C-reactive protein, in the patients with AMI. In conclusion, the serum hepcidin-20 is transiently elevated in response to acute cardiac ischemia. Measurement of serum hepcidin-20, rather than hepcidin-25, is helpful for diagnosis of acute myocardial infarction.

Low invasive angiogenic therapy for myocardial infarction by retrograde transplantation of mononuclear cells expressing the VEGF gene. Int J Cardiol. 2009 Jan 22. [Epub ahead of print]

新たな遺伝子細胞治療の共同論文発表

BACKGROUND: Although transplantation of mononuclear cells (MNCs) induces angiogenesis in myocardial infarction, transplantation requires a large amount of bone marrow or peripheral blood cells. We examined the effects of transplantation of peripheral MNCs expressing an exogenous vascular endothelial growth factor (VEGF) gene in a pig model of acute myocardial infarction (AMI). METHODS: MNCs were isolated from 20 ml peripheral blood from pigs and transfected with 10 mug of human VEGF165 plasmid (phVEGF). Myocardial infarction was induced by occlusion of the mid portion of the left anterior descending coronary artery (LAD) in anesthetized pigs. At 4 h after total occlusion, 5×10(6) VEGF-transfected MNCs were retrogradely transplanted into the pig via the coronary vein. Cardiac function, neovascularization and histology of the ischemic tissue were evaluated 4 weeks after transplantation. RESULTS: MNCs expressing hVEGF and infused via the coronary vein were efficiently delivered the heart in pigs with myocardial infarction. Transplantation of MNCs expressing hVEGF significantly increased left ventricular (LV) function, collateral vessels, and capillary density in heart from AMI model pigs. Transplantation of MNCs expressing hVEGF increased the wall thickness of the scar in the heart after AMI. CONCLUSIONS: Retrograde transplantation of peripheral blood MNCs expressing hVEGF efficiently induced angiogenesis and improved the impaired LV function in hearts of pigs with AMI. These findings indicate that angiogenic cells and gene therapy may be useful to treat ischemic heart disease.